One difficulty in deriving EpiSCs is the need for

2018-10-20

One difficulty in deriving EpiSCs is the need for microdissection of small postimplantation embryos. The original protocols used only the epiblast layer separated from the surrounding visceral endoderm (VE) tissue as a source of the cell lines. The overall efficiency of EpiSC derivation from epiblas

Here we describe the characteristics of

2018-10-20

Here, we describe the characteristics of a novel SeVdp-iPS vector (SeVdp(KOSM)302L) containing miR-302a target sequences at the 3′-UTR of the L gene. This vector can be used for the efficient reprogramming of MEFs into transgene-free iPSCs without the aid of siRNAs, observed using human embryonic fi

Expression profiles from tumor tissue and derived cell lines

2018-10-20

Expression profiles from tumor tissue and derived cell lines are extremely different, thus the current transcriptomic signature for GBM subtyping may not apply in vitro. Using our precursor state signature, we were able to identify an association with the proneural and mesenchymal subtypes. Interest

As was shown in this study along with many other

2018-10-20

As was shown in this study, along with many other previous studies, TALEN and double-nicking CRISPR/Cas9 genome-editing techniques are significantly accurate, with only a negligible risk of random gene disruption, in the drug-selected clones. This result indicates that disrupting a particular gene w

In the present study enzyme activity was dispensable for

2018-10-20

In the present study, enzyme activity was dispensable for reprogramming and iPSC maintenance. This is in contrast with Fanconi anemia (Raya et al., 2009; Navarro et al., 2014), and also with Sanfilippo type B (Lemonnier et al., 2011) and Pompe disease (Higuchi et al., 2014), in all of which gene com

The AGM explant culture is

2018-10-20

The AGM explant culture is known to increase HSC activity. Whether the increase in AGM explant HSCs is due to the expansion and shift in BMP-activation status of existing HSCs, or the new generation of HSCs (non-BMP-activated), or both, remains unclear. Secondary transplantations of GFP+ and GFP− BM

Rejection might also have occurred as iPSC CMs

2018-10-20

Rejection might also have occurred as iPSC-CMs might fail to suppress recipient NK APO866 in MHC-matched transplantation. MHC class I molecules such as MHC-E, which are expressed on almost all cells in the body, bind to receptors on NK cells to inhibit cytotoxic activity and, following the immune r

Several methods have been described to obtain neurons

2018-10-20

Several methods have been described to obtain neurons of specific subtypes through differentiation of hPSCs, either via formation of three-dimensional (3D) embryoid bodies (EBs) or using monolayers as starting material (Amoroso et al., 2013; Boissart et al., 2013; Boulting et al., 2011; Eiraku and S

Following LPS or sepsis CMP

2018-10-20

Following LPS or sepsis CMP and GMP pools are rapidly depleted, resulting in decreased output of GR1+MAC1+ cells, an effect largely prevented by loss of MYD88 but not of TRIF. Differences in integrin inhibitor and apoptosis of myeloid progenitors in the three genotypes did not sufficiently account

Previous studies of GSC reprogramming commonly utilized gene

2018-10-20

Previous studies of GSC reprogramming commonly utilized genetically modified mice. For instance, Oct4-GFP mice have been used because they provide a convenient way to monitor the process of reprogramming (Ko et al., 2010; Youn et al., 2013). Using transgenic mice may be precluded in certain research

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2018-07-29